Role for piRNAs and Noncoding RNA in de Novo DNA Methylation of the Imprinted Mouse Rasgrf1 Locus - Collections

＜journal article＞
Role for piRNAs and Noncoding RNA in de Novo DNA Methylation of the Imprinted Mouse Rasgrf1 Locus

Creator	Creator Name Watanabe, Toshiaki 渡部, 聡朗ワタナベ, トシアキ Affiliation Affiliation Name Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems \| School of Medicine, Yale University 国立遺伝学研究所人類遺伝研究部門
	Creator Name Tomizawa, Shin-ichi 富澤, 信一トミザワ, シンイチ Affiliation Affiliation Name Laboratory of Developmental Genetics and Imprinting, The Babraham Institute \| Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems 国立遺伝学研究所人類遺伝研究部門
	Creator Name Mitsuya, Kohzoh 三ツ矢, 幸造ミツヤ, コウゾウ Affiliation Affiliation Name Center for Pregnancy and Newborn Research, Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio
	Creator Name Totoki, Yasushi 十時, 泰トトキ, ヤスシ Affiliation Affiliation Name Division of Cancer Genomics, National Cancer Center Research Institute 国立がん研究センターがんゲノミクス分野
	Author PID L003698 Creator Name Yamamoto, Yasuhiro 山本, 耕裕ヤマモト, ヤスヒロ Affiliation Affiliation Name Division of Epigenomics, Medical Institute of Bioregulation, Kyushu University \| Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems 九州大学生体防御医学研究所エピゲノム学分野 \| 国立遺伝学研究所人類遺伝研究部門
	Creator Name Kuramochi-Miyagawa, Satomi 宮川（倉持）, さとみクラモチ- ミヤガワ, サトミ Affiliation Affiliation Name Graduate School of Frontier Biosciences, Osaka University 大阪大学大学院生命機能研究科
	Creator Name Iida, Naoko 飯田, 直子イイダ, ナオコ Affiliation Affiliation Name Division of Mutagenesis, Department of Molecular Genetics, National Institute of Genetics \| Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems 国立遺伝学研究所変異遺伝研究部門 \| 国立遺伝学研究所人類遺伝研究部門
	Creator Name Hoki, Yuko 保木, 裕子ホキ, ユウコ Affiliation Affiliation Name Division of Epigenomics, Medical Institute of Bioregulation, Kyushu University \| Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems 九州大学生体防御医学研究所エピゲノム学分野 \| 国立遺伝学研究所人類遺伝研究部門
	Creator Name Murphy, Patrick J. Affiliation Affiliation Name Division of Nutritional Sciences, College of Agriculture and Life Sciences, Cornell University
	Creator Name Toyoda, Atsushi 豊田, 敦トヨダ, アツシ Affiliation Affiliation Name Comparative Genomics Laboratory, Center for Genetic Resource Information, National Institute of Genetics 国立遺伝学研究所比較ゲノム解析研究室
	Creator Name Gotoh, Kengo 後藤, 健吾ゴトウ, ケンゴ Affiliation Affiliation Name Graduate School of Frontier Biosciences, Osaka University 大阪大学大学院生命機能研究科
	Creator Name Hiura, Hitoshi 樋浦, 仁ヒウラ, ヒトシ Affiliation Affiliation Name Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine 東北大学環境遺伝医学総合研究センター情報遺伝学分野
	Creator Name Arima, Takahiro 有馬, 隆博アリマ, タカヒロ Affiliation Affiliation Name Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine 東北大学環境遺伝医学総合研究センター情報遺伝学分野
	Creator Name Fujiyama, Asao 藤山, 秋佐夫フジヤマ, アサオ Affiliation Affiliation Name Principle Informatics Research Division, National Institute of Informatics \| Comparative Genomics Laboratory, Center for Genetic Resource Information, National Institute of Genetics 国立情報学研究所情報学プリンシプル研究系 \| 国立遺伝学研究所比較ゲノム解析研究室
	Author PID K003512 Creator Name Sado, Takashi 佐渡, 敬サド, タカシ Affiliation Affiliation Name Division of Epigenomics, Medical Institute of Bioregulation, Kyushu University \| Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems 九州大学生体防御医学研究所エピゲノム学分野 \| 国立遺伝学研究所人類遺伝研究部門
	Creator Name Shibata, Tatsuhiro 柴田, 龍弘シバタ, タツヒロ Affiliation Affiliation Name Division of Cancer Genomics, National Cancer Center Research Institute 国立がん研究センターがんゲノミクス分野
	Creator Name Nakano, Toru 中村, 亨ナカムラ, トオル Affiliation Affiliation Name Graduate School of Frontier Biosciences, Osaka University 大阪大学大学院生命機能研究科
	Creator Name Lin, Haifan Affiliation Affiliation Name School of Medicine, Yale University
	Author PID K003635 Creator Name Ichiyanagi, Kenji 一柳, 健司イチヤナギ, ケンジ Affiliation Affiliation Name Division of Epigenomics, Medical Institute of Bioregulation, Kyushu University \| Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems 九州大学生体防御医学研究所エピゲノム学分野 \| 国立遺伝学研究所人類遺伝研究部門
	Creator Name Soloway, Paul D. Affiliation Affiliation Name Division of Nutritional Sciences, College of Agriculture and Life Sciences, Cornell University
	Author PID K003588 Creator Name Sasaki, Hiroyuki 佐々木, 裕之ササキ, ヒロユキ Affiliation Affiliation Name Division of Epigenomics, Medical Institute of Bioregulation, Kyushu University \| Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems 九州大学生体防御医学研究所エピゲノム学分野 \| 国立遺伝学研究所人類遺伝研究部門
Language	English
Publisher	American Association for Advancement of Science
Date	2011-05-13
Source Title	Science
Vol	332
First Page	848
Last Page	852
Publication Type	Accepted Manuscript
Access Rights	open access
Related DOI	isIdenticalTo https://doi.org/10.1126/science.1203919
Related DOI	Science \|\| 332 \|\| p848-852
Related DOI	http://www.bioreg.kyushu-u.ac.jp/labo/epigenome/
Related URI	Science \|\| 332 \|\| p848-852
Related URI	http://www.bioreg.kyushu-u.ac.jp/labo/epigenome/
Related URI
Related HDL
Relation	Science \|\| 332 \|\| p848-852
Relation	http://www.bioreg.kyushu-u.ac.jp/labo/epigenome/
Abstract	Genomic imprinting causes parental origin-specific monoallelic gene expression through differential DNA methylation established in the parental germ line. However, the mechanisms underlying how specif...ic sequences are selectively methylated are not fully understood. We have found that the components of the PIWI-interacting RNA (piRNA) pathway are required for de novo methylation of the differentially methylated region (DMR) of the imprinted mouse Rasgrf1 locus, but not other paternally imprinted loci. A retrotransposon sequence within a noncoding RNA spanning the DMR was targeted by piRNAs generated from a different locus. A direct repeat in the DMR, which is required for the methylation and imprinting of Rasgrf1, served as a promoter for this RNA. We propose a model in which piRNAs and a target RNA direct the sequence-specific methylation of Rasgrf1.show more

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File	FileType	Size	Views	Description
2011-05-10	pdf	312 KB	306	プレスリリース
Watanabe4	pdf	304 KB	167	Figure4
Watanabe3	pdf	146 KB	229	Figure3
Watanabe2	pdf	127 KB	177	Figure2
Watanabe1	pdf	568 KB	269	Figure1
Text_Watanabe_070711	pdf	56.0 KB	303	本文

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Record ID	19806
Peer-Reviewed	Refereed
ISSN	0036-8075
NCID	AA00835277
Notes	九州大学生体防御医学研究所の佐々木裕之主幹教授らは、国立遺伝学研究所等との共同研究により、生殖細胞で特定の遺伝子の働きを決めるプログラミングに、piRNAとよばれる小さなRNA分子が必要であることを発見し、その機構の大筋を解明しました。新しい遺伝子調節の発見であると同時に、病気の原因となる遺伝子を抑制する技術に繋がる成果です。（九州大学プレスリリースより）
Created Date	2011.08.01
Modified Date	2024.01.10

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＜journal article＞ Role for piRNAs and Noncoding RNA in de Novo DNA Methylation of the Imprinted Mouse Rasgrf1 Locus

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＜journal article＞
Role for piRNAs and Noncoding RNA in de Novo DNA Methylation of the Imprinted Mouse Rasgrf1 Locus