作成者 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
本文言語 |
|
出版者 |
|
発行日 |
|
収録物名 |
|
巻 |
|
開始ページ |
|
終了ページ |
|
出版タイプ |
|
アクセス権 |
|
関連DOI |
|
関連DOI |
|
|
関連URI |
|
|
関連URI |
|
関連HDL |
|
関連情報 |
|
|
概要 |
Genomic imprinting causes parental origin-specific monoallelic gene expression through differential DNA methylation established in the parental germ line. However, the mechanisms underlying how specif...ic sequences are selectively methylated are not fully understood. We have found that the components of the PIWI-interacting RNA (piRNA) pathway are required for de novo methylation of the differentially methylated region (DMR) of the imprinted mouse Rasgrf1 locus, but not other paternally imprinted loci. A retrotransposon sequence within a noncoding RNA spanning the DMR was targeted by piRNAs generated from a different locus. A direct repeat in the DMR, which is required for the methylation and imprinting of Rasgrf1, served as a promoter for this RNA. We propose a model in which piRNAs and a target RNA direct the sequence-specific methylation of Rasgrf1.続きを見る
|