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The gene coding for y-polyglutamate production stimulating factor (psf) from Bacillus subtilis (natto) plasmid pUH1 was cloned and sequenced in Bacillus subtilis host. The activity of y-glutamyltransp...eptidase (r-GTP) in B. subtilis host, which was introduced the psf gene was relatively high level, while its activity in E. coli host could not be detected. The nucleotide sequence of psf gene was determined and an open reading frame encoding a plypeptide composed of 420 amino acid residues (Mr, 49, 356) was identified. The putative -35 and -10 sequences, TTCAAA and TATTAT, were observed as the consensus sequence for the promoter recognized by the σ^<43> RNA polymerase of B. subtilis, and the ribosome binding site, whose sequence was AACGAG, was complementary to the binding sequence of B. subtilis 16s rRNA except for one base. The amino acid sequence of psf with the segment of putative protein C403 of staphylococcal plasmid pE194 indicates homology, whereas that with E. coli and mammalian r-GTPs does not show any similarity at all.続きを見る
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