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| 概要 |
除草剤が水稲の根に及ぼす影響を正確かつ簡便に評価するため，種子根が均一に伸長した幼苗を用いる方法を考案し，培養液の濃度，温度条件および培養期間について検討した．さらにこの方法を用い，北部九州において使用されている主要な除草剤を評価した．その結果，培養液にセーレンゼンの燐酸塩緩衝液10倍液を用い，温度25℃で5日間培養するのが適当であると考えられた．この条件下で23種類の除草剤を検定したところ，ほと...んどの除草剤において幼芽長と冠根数は標準液の10^(-1)以下の濃度では抑制がみられなかったのに対し，種子弓長は10^(-1)以下の濃度でも抑制された．種子根長を抑制する濃度は薬剤間で異なり，標準濃度でも全く抑制しなかったのが1剤，標準濃度にのみ抑制が認められたのが1剤，10^(-1)濃度以上が4剤，10^(-2)濃度以上が11剤，10^(-3)濃度以上が4剤で，2剤には10^(-4)濃度でも有意差が認められた．
In order to evaluate simply and accurately how rice herbicides affect rice root elongation in paddy fields, we have examined the optimum culture conditions in terms of culture solution, temperature and culture duration using a root growing method in which young rice seedlings uniformly developed seminal roots. In addition, inhibition by various rice herbicides popularly used in Northern Kyushu to root elongation was evaluated using the optimum conditions. As a result, it is suggested that culture conditions were optimized for the evaluation when ten times of Sörensen's phosphate buffer solution is used as a culture solution at 25℃ for five days. Twenty-three kinds of rice herbicides were examined under these conditions. While the length of pulmuls and number of crown roots showed little inhibition in any of herbicides at 10^(-1) of standard concentration, seminal root length was inhibited in most of herbicides even at 10^(-1) or below 10^(-1) of standard concentration. Levels of the lowest concentration at which seminal root length was inhibited varied among herbicides used. Results includes one of them didn't inhibit the growth at standard concentration, one showed inhibition at standard concentration, 4 showed at 10^(-1) of standard concentration, 11 at 10^(-2), and 4 at 10^(-3). Two inhibited the growth significantly when the concentration level was set at 10^(-4).続きを見る
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