With the purpose to measure the energetic allocation that one female displays during the spawning season in iteroparous species, relationship between the egg size and its enegetic value (J/g) was measured in the anemonefish, Amphiprion clarkii. Egg dry weight was equivalent to its volume of newly laod eggs, regardless to its sizes. Eggs with different sizes had nearly the same energetic value (24.733-31.721 J/g) pre unit of its dry weight. These results showed that the total egg volume of one clutch can be regarded as the energetic allocation of the female to the clutch at the time, regardless to the number and egg sizes which differ seasonally and by female individuald.