RAPD Analysis of Genotoxic Effects of Nano–Scale SiO_2 and TiO_2 on Broad Bean (Vicia Faba L.) - Collections

＜departmental bulletin paper＞
RAPD Analysis of Genotoxic Effects of Nano–Scale SiO_2 and TiO_2 on Broad Bean (Vicia Faba L.)

Creator	Creator Name GALAL, A. Ola Affiliation Affiliation Name Department of Genetics, Faculty of Agriculture, Kafrelsheikh University
	Creator Name THABET, F. Ahmad Affiliation Affiliation Name Department of Genetics, Faculty of Agriculture, Kafrelsheikh University Affiliation Affiliation Name Laboratory of Insect Natural Enemies, Institute of Biological Control, Faculty of Agriculture, Kyushu University 九州大学大学院農学研究院資源生物科学部門 Affiliation Affiliation Name Field Crop Pests Research Department, Plant Protection Research Institute, Agricultural Research Center
	Author PID K001634 Creator Name TUDA, Midori 津田, みどりツダ, ミドリ Affiliation Affiliation Name Laboratory of Insect Natural Enemies, Institute of Biological Control, Faculty of Agriculture, Kyushu University 九州大学大学院農学研究院資源生物科学部門
	Creator Name El–SAMAHY, F.M. Magdy Affiliation Affiliation Name Field Crop Pests Research Department, Plant Protection Research Institute, Agricultural Research Center
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2020-02
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	65
Issue	1
First Page	57
Last Page	63
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/2558893

File	FileType	Size	Views	Description
p057	pdf	899 KB	545

PISSN	0023-6152
NCID	AA00697606
Record ID	2558893
Subject Terms	broad bean
	genotoxicity
	GTS
	Nano–scale materials
	RAPD
Notes	Growing use of oxide nanoparticles has driven their access to the natural environment, including biological interactions within the ecosystem, despite a lack of knowledge about their genotoxic potential on exposed plant tissue. In this study, seeds of broad beans (Vicia faba L.), one of the major carbohydrate food sources as well as an ecotoxicological model plant, were treated with three concentrations (25, 50 and 75 mg/L) of two different types of nanoscale materials (n–), n–SiO＿2 and n–TiO＿2, to assess their safe use. Seeds were soaked in n–SiO＿2 and n–TiO＿2 each for 24 h, then germinated in peat moss for two weeks. DNA was isolated from leaves for RAPD (Random Amplified Polymorphic DNA) profile analyses. Results revealed a concentration dependent genotoxic effect for n–SiO＿2 (however it seems to maintain genetic material stability) and high genotoxic effect for n–TiO＿2.
Created Date	2020.03.13
Modified Date	2023.11.20