Application of Esterase and Peroxidase Zymograms to Breeding in Brassica with Reference to Nucleus Substitution - Collections

＜departmental bulletin paper＞
Application of Esterase and Peroxidase Zymograms to Breeding in Brassica with Reference to Nucleus Substitution

Creator	Creator Name Kanazawa, Koji Affiliation Affiliation Name Laboratory of Horticulture, Faculty of Agriculture, Kyushu University
	Creator Name Eguchi, Hiromi 江口, 弘美 Affiliation Affiliation Name Biotron Institute, Kyushu University 九州大学生物環境調節研究センター
	Creator Name Iwasa, Shoichi 岩佐, 正一 Affiliation Affiliation Name Laboratory of Horticulture, Faculty of Agriculture, Kyushu University 九州大学農学部農学科園芸学講座
	Creator Name Uemoto, Shunpei 上本, 俊平 Affiliation Affiliation Name Laboratory of Horticulture, Faculty of Agriculture, Kyushu University 九州大学農学部農学科園芸学講座
Language	English
Publisher	Kyushu University
Publisher	九州大学農学部
Date	1980-08
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	25
Issue	1
First Page	25
Last Page	31
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/23717
Related DOI	九州大学農学部紀要 \|\| 25(1) \|\| p25-31
	Journal of the Faculty of Agriculture, Kyushu University \|\| 25(1) \|\| p25-31
	http://www.agr.kyushu-u.ac.jp/
Related URI	九州大学農学部紀要 \|\| 25(1) \|\| p25-31
	Journal of the Faculty of Agriculture, Kyushu University \|\| 25(1) \|\| p25-31
	http://www.agr.kyushu-u.ac.jp/
Relation	九州大学農学部紀要 \|\| 25(1) \|\| p25-31
	Journal of the Faculty of Agriculture, Kyushu University \|\| 25(1) \|\| p25-31
	http://www.agr.kyushu-u.ac.jp/

File	FileType	Size	Views	Description
p025	pdf	282 KB	409

PISSN	0023-6152
NCID	AA00697606
Record ID	23717
Peer-Reviewed	Unrefereed
Subject Terms	Brassica
	Zymograms
	Nucleus substitution
ISSN	0023-6152
NCID	AA00697606
Notes	Zymograms of leaf esterases and peroxidases were employed for the use of genetic examination in individual selection to promote nucleus substitution for breeding of new varieties, such as oleracea-cytoplasmic Brassica campestris. In successive strains derived from hybridization of B. oleracea x B. campestris and backcrosses with pollen of ssp. pekinensis, two of the esterases and one of the peroxidases were found to be specific to c genome. The pattern of the enzyme distribution was characterized by these enzymes, and there were two types of the plants in successive backcrossed strains; one had these enzymes specific to c genome, the other had none of them. Frequencies of the plants having the c genome-specific enzymes became lower according to a decrease in the number of chromosomes of c genome, in process of backcrosses with pollen of ssp. pekinensis. From this result, it was estimated that the chromosomes of c genome can be minimized by selecting the plant having none of the enzymes specific to c genome. and this method is useful for breeding of nucleus-substituted strains in Brassica.
Created Date	2012.07.31
Modified Date	2022.11.11