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＜departmental bulletin paper＞
Autoradiographic Study on the Distribution of Pregnenolone-^<14>C in Goldfish

Creator
Author PID
K001531
Creator Name
矢野, 友紀
Yano, Tomoki
Affiliation
Affiliation Name
九州大学農学部水産化学第一教室
Laboratory of Fisheries Chemistry, Faculty of Agriculture, Kyushu University
Language
Japanese
Publisher
九州大學農學部
Faculty of Agriculture, Kyushu University
Date
1979-01
Source Title
Science bulletin of the Faculty of Agriculture, Kyushu University
Vol
33
Issue
2/3
First Page
95
Last Page
100
Publication Type
Version of Record
Access Rights
open access
JaLC DOI
https://doi.org/10.15017/23272
Related DOI
九州大學農學部學藝雜誌 || 33(2/3) || p95-100
Science bulletin of the Faculty of Agriculture, Kyushu University || 33(2/3) || p95-100
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大學農學部學藝雜誌 || 33(2/3) || p95-100
Science bulletin of the Faculty of Agriculture, Kyushu University || 33(2/3) || p95-100
http://www.agr.kyushu-u.ac.jp/
Relation
九州大學農學部學藝雜誌 || 33(2/3) || p95-100
Science bulletin of the Faculty of Agriculture, Kyushu University || 33(2/3) || p95-100
http://www.agr.kyushu-u.ac.jp/

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Details

Record ID
23272
Peer-Reviewed
Unrefereed
ISSN
0368-6264
NCID
AN0005519X
Notes
In order to study on the distribution, metabolism and excretion of pregnenolone in fish, pregnenolone-<14>^C was injected into the dorsal aorta of goldfish (Carassius auratus) and after different survival times ranging from 5 minutes to 12 hours, the fish were subjected to whole-body autoradiography. The pregnenolone-<14>^C was rapidly taken up from the blood by various tissues. Five minutes after injection the highest accumulation of pregnenolone-<14>^C and/or its metabolites was observed in the kidney, gills, and muscle (Fig. 2). From 20 minutes up to 4 hours after injection, however, the highest uptake of radioactivity was registered in the liver, gall-bladder, and intestinal mucosa (Figs. 3-5). These results suggest that the liver plays an important role in steroid metabolism in fish as well as in mammals, and that steroid and/or its metabolites are excreted via the kidney, gills and gall-bladder. A specific accumulation of radioactivity was also observed in the ovary, interrenal gland, central nervous system, retina, and olfactory pits. Twelve hours after injection the radioactivity disappeared from all tissues other than retina and intestine (Fig. 6).
Pregnenoloneの魚体内分布と排泄径路を調べるために,pregnenolone-4-<14>^Cをワキン(Carrasius auratus)14尾の背大動脈に注入し,種々の生存期間(5分～12分時間)後に凍結全身オートラジオグラフィーを行なつた.Pregnenolone-4-<14>^Cは急速に血液から各種組織へ摂取された.投与後5分では腎臓,鯨,筋肉が高い放射能活性を示したが(Fig.2),投与後20分から4時間にかけては肝臓,胆のう,小腸粘膜が最大の取り込みを示した(Figs.3～5).これらの結果は魚類においても哺乳類と同様,肝臓がステロイド代謝に中心的な役割を果していることを暗示しており,またステロイドおよびその代謝産物は腎臓,鰓,胆のうから排泄されることを示唆している.上記の他,卵巣,間腎組織,中枢神経系,網膜,鼻腔にも放射能の特異的な蓄積が認められた.放射能は投与後12時間には網膜,胆のう,小腸を除くすべての魚体組織から消失した(Fig.6).
Created Date 2012.07.25
Modified Date 2022.11.11

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