A newly isolated Brevibacterium sp. strain A25R, contained about 3.3～3.7% of phosphorous compounds within cells which were significantly high as compared with those of common bacteria. Especially, the phosphorus contents of the cold acid-soluble fraction and the KOH-soluble fraction were found to be higher than those of ordinary bacteria. In the cold acid-soluble fraction, the contents of ATP and ADP were about 3.4 and 2.3 μg/mg-cells, respectively. A kind of phosphate ester of polysaccharide, phosphomannan, which could not be extracted by cold trichloroacetic acid (10%), was the main phosphorous compounds existing in the KOH-soluble fraction except for the mononucleotides originated from the decomposition of ribonucleic acid by KOH. The content of the phosphate ester was about 4.4 μg/mg-cells (as phosphorus), while the content of ribonucleic acid was about 9.3 μg/mg-cells.
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