(1) Quantitative changes of cystine, tryptophane and tyrosine in the proteins of hen's eggs during development were investigated. (2) Cystine content was determined by OKUDA'S iodine method and Folin and Looney's colorimetric method, and its increase was observed in the later period of the incubation. (3) Tryptophane content was determined by MAY and ROSE'S method with some modification. The content once increased in the middle period of the incubation and then decreased. (4) Attention was called, in the use of MAY and ROSE'S method, to determine previously the tryptophane content of casein which is used as the standard in this method. (5) Tyrosine was determined after FOLIN and DENIS. The content decreased gradually. (6) As descrived above, there were some quantitative changes of three amino-acids during incubation, but the changes were too small to conclude something from the results, when the difficulty of accurate sampling is taken into account.
1. 鷄卵發生中シスチン, トリプトファン, 及びチロシンの量的變化を考究したり。2. シスチンの定量は奧田氏の沃素法, FOLIN 及 LOONEY 氏の比色法に依れり, 後者は前者よりも稍大なる價を與ふること奧田及び元村氏の實驗に一致せり, 但し何れの方法によるもシスチンの量は鷄卵發生の後期羽毛發育の頃に於て稍増加せり。3. トリプトファンの定量は MAY 及 ROSE 氏の方法に依れり, 其量は發生中一度増加し次に減少したり。4. MAY 及 ROSE 氏法を使用する際に標準として使用するカゼイン中のトリブトファンを先づ定量し置くべき必要ある事を注意したり。5. チロシンの定量はFOLIN 及 DENIS 氏法に依れり, 此物質の量は少しく減少したり。6. 以上の如く此等三種のアミノ酸は定量の結果發生中多少増減したりと雖其増減量は甚少なるを以て, 供試品調製の困難なる事を思へば, 之によりて直に或種の斷定を與ふる事を避け單に實驗結果を報告して他日の參考に資するを安全とすべし。