1) It is found in the present experir ents that a considerable amount of transparent fluid flowed presumably out of the phallus or lymph-fold or both is added to the dense semen in vas deferens, at the moment when it is ejected; and so the ejaculated semen of a cock is considered to be a mixture of the dense semen in vas deferens and the transparent fluid. 2) The greater part of the vas deferens' semen which is able to be collected by repeated manipulations is ejected in the first manipulation, and the bulk of semen is reduced markedly to smaller amount in the following manipulations. The rate of reducton of ejected transparent fluid, however, is lower, so that, the more repeatedly the manipulations occur the more dilute the collected semen becomes. 3) There is considerable variation in the amount of ejected transparent. fluid among individuals, while slight differences in the amount of ejected semen from the vas deferens among individuals are found, and so large amount of dilute semen is collected from individuals which eject more transparent fluid. 4) It seems that the vas . deferens secretes and adds a small amount of some fluid to the semen in it, and the semen in the . vas deferens becomes gladually more dilute in successive daily collection of the semen. 5) The transparent fluid Seems to be also ejected at the time of normal eopulation.
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