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＜departmental bulletin paper＞
Production of Interspecific Germline Chimera between Quail and Chicken Utilizing the Cells from Central Disk of Blastoderm and Germinal Crescent Region

Creator
Author PID
K001589
Creator Name
Soh, Tomoki
宗, 知紀
ソウ, トモキ
Affiliation
Affiliation Name
Laboratory of Reproductive Physiology,Division of Animal Science,Department of Animal and Marine Bioresource Sciences,Faculty of Agriculture,Kyushu University
Author PID
L010442
Creator Name
Inoue, Yoshie
井上, 良枝
イノウエ, ヨシエ
Affiliation
Affiliation Name
Laboratory of Reproductive Physiology,Division of Animal Science,Department of Animal and Marine Bioresource Sciences,Graduate School of Bioresource and Bioenvironmental Sciences,Kyushu University
Creator Name
Xi, Yong Mei
Affiliation
Affiliation Name
Laboratory of Agticultural Ecology,Division of Agricuktural Ecology,Department of Plant Resources,Graduate School of Bioresource and Bioenvironmental Sciences,Kyushu University
Creator Name
Kato, Yukio
カトウ, ユキオ
Affiliation
Affiliation Name
Faculty of Agriculture,Meiji University
Creator Name
Hattori, Masa-aki
ハットリ, マサアキ
Language
English
Publisher
Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院
Date
2004-02-01
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
49
Issue
1
First Page
69
Last Page
75
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/4566
Related DOI
Journal of the Faculty of Agriculture, Kyushu University || 49(1) || p069-075
九州大学大学院農学研究院紀要 || 49(1) || p069-075
http://www.agr.kyushu-u.ac.jp/index.html
Related URI
Journal of the Faculty of Agriculture, Kyushu University || 49(1) || p069-075
九州大学大学院農学研究院紀要 || 49(1) || p069-075
http://www.agr.kyushu-u.ac.jp/index.html
Relation
Journal of the Faculty of Agriculture, Kyushu University || 49(1) || p069-075
九州大学大学院農学研究院紀要 || 49(1) || p069-075
http://www.agr.kyushu-u.ac.jp/index.html

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
4566
Peer-Reviewed
Unrefereed
ISSN
0023-6152
NCID
AA00697606
Notes
Production of interspecific germline chimera between quail and chicken was carried out using the cells from the central disk (CD) of the area pellucida in quali blastoderm (stage X) and the germinal crescent region (GCR) of quali embryo (stage7-8). The cells of CD and GCR were dispersed and injected into the subgerminal cavity of chicken blastoderm (stage X). Injected aggs were incubated for 7 days or to hatching. To detect quali genomic DNA, the polymerate chain reaction was used. In day-7 embryos, quali DNA was detected in 4 gonads and 6 brains from 55 survived embryos received quali CD cells, and 9 gonads and 8 brains from 68 survived embryos received quali GCR cells. Quali DNA was also detected from eggs or semen of adult female or male chicken hatched from eggs received quali CD or GCR cells. CD and GCR cells as the donors showed the possibility to produce the interspecific germiline chimera but the necessary improevment and the immunoreactive problem were suggested.
Created Date 2009.04.22
Modified Date 2022.11.11

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