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Metabolism of 2,2ʼ,3,4ʼ,5,6,6ʼ-Heptachlorobiphenyl (CB188) by Rat and Guinea Pig Liver Microsomes
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| Abstract | The metabolism of a 2,4,6-trichloro-substituted PCB, 2,2ʼ,3,4ʼ,5,6,6ʼ-heptachlorobiphenyl (CB188), by rat and guinea pig liver microsomes was compared to that of a 2,4,5-trichloro-substituted PCB, 2,2...ʼ,3,4ʼ,5,5ʼ,6-heptachlorobiphenyl (CB187) using liver microsomes of untreated, phenobarbital (PB)-treated and 3-methylcholanthrene (MC)-treated rats and guinea pigs. Two metabolites, M1 and M2, were produced only by liver microsomes of PB-treated rats with rates of 554 and 187 pmol/hr/mg protein, respectively, but no metabolite by liver microsomes of untreated and MCtreated rats. In addition, no metabolite was observed by all kinds of guinea pig liver microsomes used in this study. GC-MS revealed that both M1 and M2 were mono-hydroxylated CB188. Also, the methylated M1 almost completely agreed with a synthesized authentic 3ʼ-methoxy-CB188 in terms of the retention times and mass fragmentation. These results suggest that 3ʼ-OH-CB188 is a major metabolite formed by PB-inducible CYP2B enzymes in rats and that the 2,4,6-trichloro-substituted PCBs are metabolized much more easily than the 2,4,5-trichloro-substituted PCBsshow more |
| Table of Contents | はじめに 実験方法 実験結果 考察 |
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| Created Date | 2019.07.22 |
| Modified Date | 2021.03.03 |