cDNA Library Construction in the Multicolored Asian Ladybird Beetle, Harmonia axyridis (Coleoptera: Coccinellidae) for Gene Functional Analysis Using Gateway Cloning System - Collections | Kyushu University Library

＜departmental bulletin paper＞
cDNA Library Construction in the Multicolored Asian Ladybird Beetle, Harmonia axyridis (Coleoptera: Coccinellidae) for Gene Functional Analysis Using Gateway Cloning System

Creator	Creator Name JUNG, Yu Bin Affiliation Affiliation Name Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University \| Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Kyushu University
	Creator Name KIM, Jeong Hee Affiliation Affiliation Name Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University \| Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Kyushu University
	Creator Name KWON, Hye Ri Affiliation Affiliation Name Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University \| Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Kyushu University
	Creator Name LIM, Hyoun Sub Affiliation Affiliation Name Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University \| Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Kyushu University
	Creator Name YU, Yong Man Affiliation Affiliation Name Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University \| Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Kyushu University
	Author PID K001619 Creator Name YASUNAGA–AOKI, Chisa 青木, 智佐アオキ, チサ Affiliation Affiliation Name Department of Bio Environmental Chemistry, College of Agricultural Biology and Life Sciences, Chungnam National University \| Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Kyushu University 九州大学大学院農学研究院資源生物科学部門 : 教授
	Creator Name YOUN, Yong Man Affiliation Affiliation Name Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University \| Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Kyushu University
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2018-09-01
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	63
Issue	2
First Page	281
Last Page	292
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/1955397

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PISSN	0023-6152
NCID	AA00697606
Record ID	1955397
Subject Terms	cDNA library
	Ecdysone receptor
	Gateway cloning system
	Harmonia axyridis
Notes	In this preliminary study, we sought to investigate the genes involved in the differentiation of elytral color and pattern in the beetle Harmonia axyridis. We confirmed the inhibition of ecdysis after knocking down H. axyridis ecdysone receptor A, and generated an H. axyridis cDNA library using the Gateway system for functional analysis of undiscovered genes in H. axyridis. We generated an H. axyridis. cDNA library with a titer of 4.43 × 104 and insert sizes of approximately 200–700 bp. Examination of the genetic information of this cDNA library showed that most sequences were from insect genes and one was an H. axyridis gene. After cloning H. axyridis ecdysone receptor A into a LITMUS 28i vector, 400–500 bp double– stranded RNA was synthesized using the T7 promoter. When the synthesized double–stranded ecdysone receptor A was injected between the 1st and 2nd sections of the abdomen of 0 to 2–day–old 4th–instar larvae of H. axyridis, most of the larvae died within 4 days after injection, whereas others failed to pupate. qRT– PCR analysis showed that, compared with non–treatment and distilled water treatment groups, the expression level of ecdysone receptor A in larvae injected with double–stranded RNA of ecdysone receptor A decreased markedly at day 4 after injection.
Created Date	2018.09.04
Modified Date	2022.11.11