Efficient in vitro Plant Regeneration from Protocorm of Bletilla formosana (Hayata) Schltr. - Collections | Kyushu University Library

＜departmental bulletin paper＞
Efficient in vitro Plant Regeneration from Protocorm of Bletilla formosana (Hayata) Schltr.

Creator	Creator Name NGUYEN, Minh–Khiem Affiliation Affiliation Name Faculty of Applied Sciences, Ton Duc Thang University \| Laboratory of Horticultural Science, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University
	Creator Name LIN, Kuan–Hung Affiliation Affiliation Name Department of Horticulture and Biotechnology, Chinese Culture University \| Laboratory of Horticultural Science, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University
	Creator Name CHEN, Li–Ru Affiliation Affiliation Name Department of Horticulture and Biotechnology, Chinese Culture University \| Laboratory of Horticultural Science, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University
	Creator Name HSIUNG, Tung–Chuan Affiliation Affiliation Name Department of Horticulture and Biotechnology, Chinese Culture University \| Laboratory of Horticultural Science, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University
	Author PID K001614 Creator Name OZAKI, Yukio 尾崎, 行生オザキ, ユキオ Affiliation Affiliation Name Laboratory of Horticultural Science, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University 九州大学大学院農学研究院資源生物科学部門 : 教授
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2018-09-01
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	63
Issue	2
First Page	231
Last Page	240
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/1955387

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PISSN	0023-6152
NCID	AA00697606
Record ID	1955387
Subject Terms	Bletilla formosana
	multiple shoots
	nitrogen content
	plant growth regulators
	protocorm
Notes	An efficient mass propagation method for Bletilla formosana (Hayata) Schltr. was successfully established through direct shoot organogenesis. Multiple shoots were induced from protocorm explants on half–strength Murashige and Skoog (1/2–MS) basal medium containing 2 mg/L N–phenyl–N’–1,2,3–thiadiazol–5–yl urea (TDZ) and 0.5 mg/L 1–naphthaleneacetic acid (NAA) with the highest shoot formation rate of 100% and a maximum average shoot number of 5.2. The application of either NAA or 2,4–dichlorophenoxy (2,4–D) significantly induced root induction from shoots, and 2 mg/L NAA provided the highest root formation rate of 100% and a maximum number of roots (4.4 per shoot). Supplementation with kinetin and benzylaminopurine (BAP) of 0.5~2.0 mg/L in 1/2–MS basal medium significantly promoted plantlet growth. To optimize the in vitro development of plantlets, 1/2–MS basal medium with modified 1/4–strength nitrogen content, and 20 g/L sucrose was used. Well–developed plantlets were successfully acclimatized in a greenhouse with over a 90% survival rate. This effective protocol of in vitro plant regeneration through direct shoot organogenesis can be utilized for the mass propagation and germplasm conservation of B. formosana
Created Date	2018.09.04
Modified Date	2022.11.11