Occurrence of Spontaneous Polyembryony in Lilium lancifolium Thunb. - Collections | Kyushu University Library

＜departmental bulletin paper＞
Occurrence of Spontaneous Polyembryony in Lilium lancifolium Thunb.

Creator	Creator Name Ye–Su, Song Affiliation Affiliation Name Department of Horticultural Science, Kangwon National University \| Oriental Bio–herb Research Institute, Kangwon National University \| Laboratory of Horticultural Science, Department of Agro–environmental Sciences, Faculty of Agriculture, Kyushu University
	Creator Name Kim, Jong–Hwa Affiliation Affiliation Name Department of Horticultural Science, Kangwon National University \| Oriental Bio–herb Research Institute, Kangwon National University \| Laboratory of Horticultural Science, Department of Agro–environmental Sciences, Faculty of Agriculture, Kyushu University
	Author PID K001672 Creator Name Wakana, Akira 若菜, 章 Affiliation Affiliation Name Laboratory of Horticultural Science, Department of Agro–environmental Sciences, Faculty of Agriculture, Kyushu University 九州大学大学院農学研究院資源生物科学部門 : 准教授
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2017-09-08
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	62
Issue	2
First Page	345
Last Page	351
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/1854005

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PISSN	0023-6152
NCID	AA00697606
Record ID	1854005
Peer-Reviewed	Unrefereed
Subject Terms	polyspermy
	SSR marker
	Tiger lily
	triplet embryos
	twin embryos
ISSN	0023-6152
NCID	AA00697606
Notes	We examined spontaneous polyembryony in Lilium lancifolium Thunb. by determining the occurrence of polyembryonic seeds in intraspecific crosses and open pollination, and by analyzing the genetic origins of polyembryos with simple–sequence repeat (SSR) markers. Forty–one polyembryonic seeds (0.2% of total) were detected from 17,583 seeds. Of the polyembryonic seeds, 36 were duplets and five were triplets. Eighteen seedling sets (three triplets and 15 duplets) were cultured and grown to maturity. Flow cytometry revealed that all were diploids, and results of analyses with SSR markers indicated that all the multiple embryos, except for two triplet seeds, originated from a zygotic embryo through cleavage embryony. The SSR profiles confirmed that the seedlings from polyembryos resulted from cross–fertilizations and did not from unfertilized somatic embryos. Six seedlings rescued from the two triplets occurring in 2x × 3x combinations were heterozygous with respect to SSR, and thus they may have their origin in multiple embryo sacs and/or polyspermy. The occurrence of polyembryonic seeds was remarkably greater in one accession collected from Wan–do (Wan island) than the others. This suggested that the frequency of polyembryony depends on the genotypes of L. lancifolium.
Created Date	2017.10.04
Modified Date	2022.11.11