An Efficient Protocol of Protocorm–Like Bodies Regeneration from Callus Cultures of Gastrodia elata Blume and the Further Associations with Mycorrhizal Fungi - Collections

＜departmental bulletin paper＞
An Efficient Protocol of Protocorm–Like Bodies Regeneration from Callus Cultures of Gastrodia elata Blume and the Further Associations with Mycorrhizal Fungi

Creator	Creator Name Yeh, Chih-Hsin Affiliation Affiliation Name Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University \| Taoyuan District Agricultural Research and Extension Station, Council of Agriculture
	Creator Name Liao, Fang-Shin Affiliation Affiliation Name Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University \| Taoyuan District Agricultural Research and Extension Station, Council of Agriculture
	Creator Name Huang, Kuang-Liang Affiliation Affiliation Name Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University \| Department of Horticultural Science, National Chiayi University
	Author PID K002580 Creator Name Miyajima, Ikuo 宮島, 郁夫ミヤジマ, イクオ Affiliation Affiliation Name Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University 九州大学熱帯農学研究センター
	Creator Name Lee, Yung-I Affiliation Affiliation Name Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University \| Biology Department, National Museum of Natural Science \| Department of Life Sciences, National Chung Hsing University
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2017-02-24
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	62
Issue	1
First Page	39
Last Page	46
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/1798139

File	FileType	Size	Views	Description
p039	pdf	2.48 MB	710

PISSN	0023-6152
NCID	AA00697606
Record ID	1798139
Peer-Reviewed	Unrefereed
Subject Terms	Armillaria
	liquid culture
	mycoheterotrophic orchid
	mycorrhizal fungi
	somatic embryo
ISSN	0023-6152
NCID	AA00697606
Notes	In this study, we reported a reliable protocol of PLBs regeneration from embryogenic callus cultures of Gastrodia elata, a fully mycoheterotrophic orchid and an endangered herbal medicinal plant. The continuous immersion cultures with TDZ (0.5 and 1 mg · L^〈–1〉) and BA (10 mg · L^〈–1〉) improved PLBs regeneration frequency and rate. Histological studies indicated that starch grains were abundant within in the cytoplasm of embryogenic callus and developing PLBs. The formation of PLBs from cell aggregates in the periphery region of embryogenic callus was supposed to be the multicellular origin pathway. After inoculated with suitable Armillaria isolates, PLBs enlarged further and resulted in the formation of tubers. The results of phylogenetic analysis and inoculation experiments indicated that the asymbiotic PLBs could establish the appropriate association with Armillaria mellea complex, including A. mellea and A. gallica. Thus, this protocol of PLBs regeneration from callus cultures is beneficial for the multiplication of selected elites, and could be used as an experimental system for the molecular investigations of the mycoheterotrophic orchid – mycorrhizal fungi interactions.
Created Date	2017.03.15
Modified Date	2022.11.11