Contamination and survival of Salmonella on leaf vegetable during cultivation - Collections | Kyushu University Library

＜departmental bulletin paper＞
Contamination and survival of Salmonella on leaf vegetable during cultivation

Creator	Creator Name 三島, 朋子 Mishima, Tomoko ミシマ, トモコ Affiliation Affiliation Name 九州大学大学院生物資源環境科学府生命機能科学専攻食料化学工学教育コース食品衛生化学研究室 Laboratory of Food Hygienic Chemistry, Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
	Creator Name 島本, 美紗子 Shimamoto, Misako シマモト, ミサコ Affiliation Affiliation Name 九州大学大学院生物資源環境科学府生命機能科学専攻食料化学工学教育コース食品衛生化学研究室 Laboratory of Food Hygienic Chemistry, Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
	Creator Name 城戸, 希望 Kido, Nozomi キド, ノゾミ Affiliation Affiliation Name 九州大学大学院生物資源環境科学府生命機能科学専攻食料化学工学教育コース食品衛生化学研究室 Laboratory of Food Hygienic Chemistry, Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
	Author PID K001621 Creator Name 本城, 賢一 Honjoh, Ken-ichi ホンジョウ, ケンイチ Creator Alternative Name Honjo, Kenichi Affiliation Affiliation Name 九州大学大学院農学研究院生命機能科学部門食料化学工学講座食品衛生化学研究室 Laboratory of Food Hygienic Chemistry, Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
	Author PID K001577 Creator Name 宮本, 敬久 Miyamoto, Takahisa ミヤモト, タカヒサ Affiliation Affiliation Name 九州大学大学院農学研究院生命機能科学部門食料化学工学講座食品衛生化学研究室 Laboratory of Food Hygienic Chemistry, Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
Language	Japanese
Publisher	九州大学大学院農学研究院
Publisher	Faculty of Agriculture, Kyushu University
Date	2016-09-01
Source Title	Science bulletin of the Faculty of Agriculture, Kyushu University
Vol	71
Issue	2
First Page	37
Last Page	45
Publication Type	Version of Record
Access Rights	open access
JaLC DOI	https://doi.org/10.15017/1687703
Table of Contents	緒言材料と方法結果および考察

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PISSN	1347-0159
NCID	AA11577672
Record ID	1687703
Peer-Reviewed	Unrefereed
Subject Terms	汚染土壌
	contaminated soil
	汚染潅水
	contaminated water
	サルモネラ
	Salmonella
	ホウレンソウ
	Spinach
Notes	Salmonella Enteritidis に緑色蛍光タンパク質(GFP)発現プラスミドを導入したS. Enteritidis-GFPを約2，4，6，8 logCFU/gとなるように接種した初期汚染土壌でサラダ用ホウレンソウを栽培して調べた結果，播種から35 日目の収穫時には初期汚染濃度に比例して4〜7 logCFU/gのS. Enteritidis-GFPが土壌中に生残した．このS. Enteritidis-GFP初期汚染土壌で栽培・収穫したホウレンソウ可食部の汚染を調べた結果，3.8 logCFU/g以下の汚染土壌で栽培したホウレンソウは，すべて定性試験でS. Enteritidis-GFP陰性であったが，6.0 logCFU/g 以上の初期汚染土壌では全てS.Enteritidis-GFP陽性であった．しかし，収穫後に可食部分を表面殺菌して調べた結果、全て陰性となった．S. Enteritidis-GFP 汚染水を頭上潅水した結果、3.1 logCFU/mL以下のS. Enteritidis-GFP汚染水では潅水から7 日後には試験した18 検体すべてS. Enteritidis-GFP陰性であった．
Notes	Salmonella contamination of spinach leaf during cultivation was investigated. Salmonella Enteritidis expressing green fluorescent protein（S . Enteritidis-GFP）was used in this study. S . Enteritidis-GFP was detected from soil, which had been initially inoculated with the bacterium at more than 2 logCFU/g after 35-d cultivation. The viable S . Enteritidis-GFP counts in soil ranged from 4 to 7 logCFU/g in proportion to the initial inoculum from 2 to 8 logCFU/g after 35-d cultivation. All the 6 spinach plants were negative for S . Enteritidis-GFP by the qualitative test, when spinach had been cultivated in a soil initially contaminated with the bacterium at less than 3.8 logCFU/g. In contrast, all the 6 plants were positive for the bacterium when cultivated in a soil contaminated with more than 6.0 logCFU/g. However, the leaves of all 9 plants were negative for S . Enteritidis-GFP after surface sterilization, suggesting the surface contamination. At 7d after overhead irrigation of water contaminated with S . Enteritidis- GFP at less than 3.1 logCFU/mL, all the spinach plants were negative for the bacterium in 18 separately cultivated spinach plants.
Created Date	2016.10.21
Modified Date	2022.11.11