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＜departmental bulletin paper＞
Molecular Diagnosis of the Biological Control Agent Nesidiocoris tenuis (Tobacco Plant Bug) and Its Allied Species (Insecta: Hemiptera) Using COI Barcoding

Creator
Creator Name
Kim, Junggon
Affiliation
Affiliation Name
Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University
Creator Name
Lee, Hodan
Affiliation
Affiliation Name
Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University
Creator Name
Lee, Wang Hee
Affiliation
Affiliation Name
Department of Biosystems Machinery Engineering, College of Agriculture and Life Sciences, Chungnam National University
Creator Name
Yu, Yong Man
Affiliation
Affiliation Name
Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University
Author PID
K001619
Creator Name
Yasunaga-Aoki, Chisa
Affiliation
Affiliation Name
Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Kyushu University
Author PID
K001619
Creator Name
Yasunaga, Chisa
安永, 智佐
ヤスナガ, チサ
Affiliation
Affiliation Name
Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院附属生物的防除研究施設天敵微生物学部門
Author PID
K001619
Creator Name
Aoki, Chisa
青木, 智佐
アオキ, チサ
Affiliation
Affiliation Name
Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院附属生物的防除研究施設天敵微生物学部門
Creator Name
Jung, Sunghoon
Affiliation
Affiliation Name
Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University
Language
English
Publisher
Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院
Date
2016-09-01
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
61
Issue
2
First Page
293
Last Page
297
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/1685885

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
1685885
Peer-Reviewed
Unrefereed
Subject Terms
Barcoding
Biological control agent
COI
Diagnosis
Miridae
Nesidiocoris tenuis
ISSN
0023-6152
NCID
AA00697606
Notes
DNA barcoding is a useful molecular method for identification of certain animal groups. It uses partial DNA sequences of mitochondrial genes such as the cytochrome c oxidase subunit I (COI) gene. In this study, effectiveness of the COI barcoding as an identification tool for Nesidiocoris tenuis, which is used as a biological control agent, and for its allied species, was evaluated. All the species used in this study had a distinct COI barcode sequence, and the Neighbor–Joining (NJ) tree based on COI sequences almost matched the morphological classification for most genera investigated in this study, except for the genus Cyrtopeltis. The average interspecific genetic distance between N. tenuis and its allied species was 111 times higher than the average intraspecific genetic distance. The tree showed shallow intraspecific divergences and deep interspecific divergences. Therefore, our results suggested that COI barcode for N. tenuis and its allied species can be used as an effective identification tool by entomologists, quarantine experts and other related researchers, and can provide directions to taxonomists for further taxonomic studies.
Created Date 2016.10.07
Modified Date 2022.11.11

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