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＜departmental bulletin paper＞
The Large Scale Production of Cerrena unicolor Laccase on Waste Agricultural Based Media

Creator
Creator Name
Mazur, Iwona
Affiliation
Affiliation Name
Department of Biochemistry, Maria Curie–Skłodowska University
Creator Name
Rola, Beata
Affiliation
Affiliation Name
Department of Biochemistry, Maria Curie–Skłodowska University
Creator Name
Stolarczyk, Krzysztof
Affiliation
Affiliation Name
Faculty of Chemistry, University of Warsaw
Creator Name
Nazaruk, Ewa
Affiliation
Affiliation Name
Faculty of Chemistry, University of Warsaw
Creator Name
Bilewicz, Renata
Affiliation
Affiliation Name
Faculty of Chemistry, University of Warsaw
Creator Name
Rogalski, Jerzy
Affiliation
Affiliation Name
Department of Biochemistry, Maria Curie–Skłodowska University | Laboratory of Forest Resources Management, Division of Forest Environmental Sciences, Department of Agro–environmental Sciences, Faculty of Agriculture, Kyushu University
Creator Name
Ohga, Shoji
大賀, 祥治
オオガ, ショウジ
Affiliation
Affiliation Name
Laboratory of Forest Resources Management, Division of Forest Environmental Sciences, Department of Agro–environmental Sciences, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院環境農学部門森林環境科学講座
Language
English
Publisher
Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院
Date
2015-09-18
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
60
Issue
2
First Page
297
Last Page
302
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/1526341
Related DOI
http://www.agr.kyushu-u.ac.jp/
Related URI
http://www.agr.kyushu-u.ac.jp/
Relation
http://www.agr.kyushu-u.ac.jp/

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
1526341
Peer-Reviewed
Unrefereed
Subject Terms
Cerrena unicolor
laccase
culture factors
wood degrading
fermentor
ISSN
0023-6152
NCID
AA00697606
Notes
The effect of different agriculture waste polysaccharides as an only carbon source in culture medium of wood–degrading basidiomycete Cerrena unicolor C–139 were investigated. The maximal growth and laccase synthesis in shaken flask was observed in mineral salts broth containing potato starch as the carbon source and asparagine as the nitrogen source (11,000 nkat/L). When an optimized medium was stimulated by addition of 10 μM Cu^<2+> to the culture medium on days 3–5 the maximal activity 24,000 nkt/L was obtained. Next the influence of the stabilization of the medium pH after 48–h incubation on laccase activity in fermentor cultures had to be estimated. The obtained data show that use of an automatic pH control set at pH 5.0 increased laccase productivity significantly (by 12 times) as compared to that obtained in the fermentor culture with a non–stabilized pH–value. Under the new conditions, the highest enzyme activity of 290,000 nkat/L was reached after 13–day incubation.
Created Date 2015.10.15
Modified Date 2022.11.11

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