Optimization of Medium for the Production of a Novel Aquaculture Probiotic, Streptomyces sp. A1 Using Central Composite Design of Response Surface Methodology - Collections

＜departmental bulletin paper＞
Optimization of Medium for the Production of a Novel Aquaculture Probiotic, Streptomyces sp. A1 Using Central Composite Design of Response Surface Methodology

Creator	Creator Name Chau, Ngo Thi Tuong Affiliation Affiliation Name Ha Noi University of Science, Viet Nam National University
	Author PID K001636 Creator Name Matsumoto, Masaru 松元, 賢マツモト, マサル Affiliation Affiliation Name Institute of Tropical Agriculture, Kyushu University 九州大学熱帯農学研究センター
	Author PID K002580 Creator Name Miyajima, Ikuo 宮島, 郁夫ミヤジマ, イクオ Affiliation Affiliation Name Institute of Tropical Agriculture, Kyushu University 九州大学熱帯農学研究センター
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2014-02-28
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	59
Issue	1
First Page	25
Last Page	32
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/1434376
Related DOI	http://www.agr.kyushu-u.ac.jp/
Related URI	http://www.agr.kyushu-u.ac.jp/
Relation	http://www.agr.kyushu-u.ac.jp/

File	FileType	Size	Views	Description
p025	pdf	3.61 MB	906

PISSN	0023-6152
NCID	AA00697606
Record ID	1434376
Peer-Reviewed	Unrefereed
Subject Terms	aquaculture probiotic
	central composite design
	Streptomyces sp. A1
	Vibrio harveyi V7
Notes	A strain, Streptomyces sp. A1 isolated from shrimp pond sediments has been identified as an aquaculture probiotic antagonistic to pathogenic Vibrio harveyi V7. In the present study, the different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity, following response surface methodology (RSM). Accordingly, the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design (CCD) of RSM for the final optimization. The response surface plots of biomass production showed similar pattern with that of antagonistic activity, which indicated a correlation between the biomass and antagonism. The optimum concentrations of the carbon and nitrogen source, and growth factors for both biomass and antagonistic activity were starch (14.62 g/l), casein (0.93 g/l), NaCl (15.6 g/l), DL–α–alanine (0.233 g/l), and vitamin B_6 (0.023 g/l).
Created Date	2014.04.07
Modified Date	2022.11.11