<博士論文>
遺伝子量補償の破たんはマウス栄養膜細胞における遺伝子発現制御に広範な影響を及ぼす

作成者
論文調査委員
本文言語
学位授与年度
学位授与大学
学位
学位種別
出版タイプ
アクセス権
JaLC DOI
関連DOI
概要 Xist RNA, which is responsible for X inactivation, is a key epigenetic player in the embryogenesis of female mammals. Of the several repeats conserved in Xist RNA, the A-repeat has been shown to be es...sential for its silencing function in differentiating embryonic stem cells. Here, we introduced a new Xist allele into mouse that produces mutated Xist RNA lacking the A-repeat (Xist^<CAGΔ5'> ). Xist^<CAGΔ5'> RNA expressed in the embryo coated the X chromosome but failed to silence it. Although imprinted X inactivation was substantially compromised upon paternal transmission, allele-specific RNA-seq in the trophoblast revealed that Xist^<CAGΔ5'> RNA still retained some silencing ability. Furthermore, the failure of imprinted X inactivation had more significant impacts than expected on genome-wide gene expression. It is likely that dosage compensation is required not only for equalizing X-linked gene expression between the sexes but also for proper global gene regulation in differentiated female somatic cells.続きを見る

本文ファイル

med3065 pdf 10.7 MB 419 本文
med3065_abstract pdf 174 KB 82 要旨
med3065_summary pdf 174 KB 143 要約
med3065_review pdf 170 KB 96 審査結果要旨

詳細

レコードID
査読有無
権利関係
関連PubMed ID
報告番号
学位記番号
授与日(学位/助成/特許)
受理日
部局
登録日 2018.05.30
更新日 2020.01.31

この資料を見た人はこんな資料も見ています