Seabuckthorn （Hippophae rhamnoides L.） is the plant which contained many nutrients, for vitamin. In this study, the effect of seabuckthorn lees （SL） which added in concentrate feed on the growth and blood parameter, in particular factors in relation to stress in the weaning period was investigated using Japanese black cattle （n＝15）. Control group （n＝5, body weight: 81.1±4.6kg） , 5％ SL group （n＝5, 85.4±9.3kg） and 10 ％ SL group （n＝5, 80.2±6.4kg） were set up. The changes of body weight and blood parameter from wean to six months cattle were investigated. Body weight in 5 ％ and 10 ％ SL group were lower （P＜0.05） than that of control group. There were differences of serum total protein, albumen and triglyceride among group. Contents of γ-GTP in 5％ group was higher （P＜0.05） those of control and 10 ％ group from weaning to one month. The contents of IgA, IgG and IgM were not changed. Therefore growth and blood parameter in cattle were not affected by the addition to SL （within 10 ％）. However, body weight in cattle decreased for SL addition. For use of SL, future study needs to investigate the processing and extracting of SL.