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Leaf genomic DNA, leaf and pollen poly (A+) -RNA preparations have been extracted from ryegrass, Lolium perenne L. grown under green-house conditions. A 790-nt fragment has been identified with a spec...ific degenerative primer pair to the conserved 3'-terminal region of putative S-gene of Phalaris coerulescens L. using PCR of total DNA purified from self-incompatible mutants. A 214-nt amplification product has been detected using same specific primers in course of RT-PCR. Sizes of DNA and cDNA fragments generated appeared to be in a strict conformity with corresponding sequences earlier established for the pollen of other cereals, such as Phalaris coerulescens L. and Secale cereale L. Thioredoxin H-like sequence and its product is thus likely to be either essential for ryegrass self-incompatibility, moreover that multifunctional behaviour of the protein might also contribute to corporative interaction of tissue-unspecific, ubiquitous genes at flowering.続きを見る
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Mendeley出力
KJ00004506895