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| 概要 |
Recent studies show that during slow freezing of cells, in addition to damage by so-called solution effect, the cells may be also injured by mechanical damage induced by ice crystal compression. A new... experimental procedure was employed to develop a quantitative understanding of cell destruction by deformation with two parallel surfaces. The viability of deformed cells (prostatic carcinoma cell line PC-3) was measured at 0℃, 23℃ and 37℃, and six different nominal gaps between 32.3μm and 3.5μm. The relation between measured viability and gap size for 0℃ and 23℃ are identical. This may suggest that deformation damage is not related to the mechanical properties of the lipid membrane but the deformation of the cytoskelton.続きを見る
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Mendeley出力
KJ00004507929