Liposomal microcapsulation containing enzymes were prepared by proliposome method to improve a stability of enzymes under acidic conditions. Two model enzymes, β-galactosidase and alkaline phosphatase, were encapsulated by a method with soybean lecithin (β-galactosidase; 57.3%, alkaline phosphatase; 53.0%) . However, the microcupsules did not exhibit the stabilizing effect for β-galactosidase at pH 3. Chitosan-coating was made for proloposome-capsules to keep enzymatic activity under acidic conditions. The mictocupsules coated with >0.5% of chitosan 10 showed high stability at pH3, and encapsulated β-galactosidase was retained 80.7% of its activity. Similarly, alkaline phosphatase was highly stabilized by the encapsulation with chitosan-coating at pH5 for 60min, while uncapsulated one lost 53.3% of its activity. The activity of encapsulated alkaline phosphatase was kept 20.1 and 59.2% for 60 min at pH3 and 4, respectively, while uncapsulated alkaline phosphatase was completely inactivated at these pHs.
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