In vitro morphogenesis from pedicels of Hippeastrum x hybridum cv. Hermitage was investigated. Bulblet formation rates and number of bulblets were excellent when the concentrations of naphthyl acetic acid (NAA) and N[6]-benzyladenine (BA) were the same. The combination of 5mgl[-1] 2,4-dichlorophenoxyacetic acid (2,4-D) with 0.002-2mgl[-1] thidiazuron (TDZ) induced somatic embryogenesis with high rate and the combination of 5mgl[-1] 2,4-D and 2mgl[-1] TDZ gave the greatest number of somatic embryos among the treatments. High concentrations of TDZ (0.2-2mgl[-1]) promoted bulblet formation, but reduced root formation. Number of shoot primordia was high with higher 2,4-D concentrations irrespective of TDZ. The highest number of bulblets (6.4) was obtained with 1mgl[-1] 2,4-D+0.2mgl[-1] TDZ.
Mendeley出力
