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＜紀要論文＞
Characterization of the Soluble Membrane Attack Complex (SMAC) of Carp (Cyprinus carpio) Complement

作成者
著者識別子
K001611
作成者名
Nakao, Miki
中尾, 実樹
ナカオ, ミキ
所属機関
所属機関名
Laboratory of Marine Biochemistry,Division of Marine Biological Chemistry,Department of Bioscience and Biotechnology,Faculty of Agriculture,Kyushu University
作成者名
Uemura, Takeshi
ウエムラ, タケシ
所属機関
所属機関名
Biocheimstry,Division of Marine Biological Chemistry,Department of Bioscience and Biotechnology,Graduate School of Bioresource and Bioenvironmental Sciences,Kyushu University | Laboratory of Marine Biochemistry,Division of Marine Biological Chemistry,Department of Bioscience and Biotechnology,Faculty of Agriculture,Kyushu University
著者識別子
K001531
作成者名
Yano, Tomoki
矢野, 友紀
ヤノ, トモキ
本文言語
英語
出版者
Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院
発行日
2003-10-01
収録物名
九州大学大学院農学研究院紀要
巻
48
号
1/2
開始ページ
127
終了ページ
134
出版タイプ
Version of Record
アクセス権
open access
Crossref DOI
https://doi.org/10.5109/4535
関連DOI
Journal of the Faculty of Agriculture, Kyushu University || 48(1/2) || p127-134
九州大学大学院農学研究院紀要 || 48(1/2) || p127-134
http://www.agr.kyushu-u.ac.jp/index.html
関連URI
Journal of the Faculty of Agriculture, Kyushu University || 48(1/2) || p127-134
九州大学大学院農学研究院紀要 || 48(1/2) || p127-134
http://www.agr.kyushu-u.ac.jp/index.html
関連情報
Journal of the Faculty of Agriculture, Kyushu University || 48(1/2) || p127-134
九州大学大学院農学研究院紀要 || 48(1/2) || p127-134
http://www.agr.kyushu-u.ac.jp/index.html

本文ファイル

pdf p127 pdf 584 KB 339  

詳細

PISSN
0023-6152
NCID
AA00697606
レコードID
4535
査読有無
査読無
ISSN
0023-6152
NCID
AA00697606
注記
The membrane attack complex (MAC) of mammalian complement, which is composed of complement components,C5b, C6, C7, C8 and C9, shows cytolytic activity by distributing the structure of cytoplasmic mambrane. The soluble form of the membtane attack complex, termed SMAC, of the complement system is innert unlike the membtane attack complex formed on target cell membranes. Thus formation of SMAC is inferred as a regulatory mechanism of th cytotoxic activity of the complement system. In the present study, a complex homologous to mammalian SMAC was purified from zymosan-activated carp serum by gel filtration through Sepharose CL-6B and affinity chromatography using anti-carp C9-Toyopearl. The purified carp SMAC showed a partial antigenicity of C9 and behaved as a 1,020 kDa α-globulin. Two-dimensional SDS-PAGE of carp SMAC recealed polypeptide spots of C5b, C6, C7,C8 and Cp, in agreement with those previously identified in carp MAC. In addition, three spots specific to SMAC were detected on the two-demensional gel, which may represent proteins responsible for solubilization of MAC, such as SP40,40 and S-protein present in mammalian SMAC. These results suggest that structure and function of SMAC have been conserved through the evolution of vertebrates.
登録日 2009.04.22
更新日 2022.11.11

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