Laboratory of Horticultural Science, Division of Agricultural Botany, Department of Plant Resources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
Shoot formatioin from rhizome apicies of Cymbidium ensifolium was promoted on Murashige and Skoog (MS) medium supplemented with 1 or 10 mgl[-1] with or without NAA, and on MS medium with the reduction of ammonium and potassium nitrates to 25 and 50%, respectively from their original concentrations (modified MS medium, MMS). The optimal time and length of culture of C. ensifolium and C. kanran on MMS or MS medium with 1mgl[-1] BA and 0.1mgl[-1] NAA (BN medium) for shoot formatioin were when the explans were cultures for at least one month and at about two months after the initial culture on MS medium, respectively.
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