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Shoot formatioin from rhizome apicies of Cymbidium ensifolium was promoted on Murashige and Skoog (MS) medium supplemented with 1 or 10 mgl[-1] with or without NAA, and on MS medium with the reduction... of ammonium and potassium nitrates to 25 and 50%, respectively from their original concentrations (modified MS medium, MMS). The optimal time and length of culture of C. ensifolium and C. kanran on MMS or MS medium with 1mgl[-1] BA and 0.1mgl[-1] NAA (BN medium) for shoot formatioin were when the explans were cultures for at least one month and at about two months after the initial culture on MS medium, respectively.続きを見る
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