Laboratory of Horticultural Science, Division of Agricultural Botany, Department of Plant Resources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
In vitro multiplication of Alocasia micholitziana 'Green Velvet', Alocasia×amazonica and Alocasia cuculata was achieved from axillary bunds excised from the cormes of greenhouse-grown plants. Shoots were developed directly within 6-8 weeks on the MS medium supplemented with 10mgl[-1] 2-iP for A. micholitziana 'Green Velvet' or 10mgl[-1] BA for A. ×amazonica and A. cuculata. Considering the quality of the shoots, high frequency of shoot regeneration was achieved on similar medium in subsequent sebcultures for A. micholitziana 'Green Velvet' (5.0 shoots/calture) . Five mgl[-1] BA gave the best shoot regeneration in A. × amazonica and A. cuculata (4.6 and 5.0 shoots/culture, respectively). Acclimatization was successful with approximately 100% of the in vitro plantlets which survived in a greenhouse. The regenerated plants seemed morphologically to be similar to the respective mother plants. The established procedure provided a basic technique to carry out the future in vitro experiments in Alocasia.
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