作成者 |
|
|
|
|
|
|
|
本文言語 |
|
出版者 |
|
|
発行日 |
|
収録物名 |
|
巻 |
|
号 |
|
開始ページ |
|
終了ページ |
|
出版タイプ |
|
アクセス権 |
|
Crossref DOI |
|
関連情報 |
|
|
|
概要 |
Protoplasts from Chlorella vulgaris K-73112 were obtained by enzymatic digestion with a mixture of Acromopeptidase, Cellulase ONOZUKA R-10, Chitosanas Kl, Gluczyme, and Uskizyme. The formation of nake...d protoplasts was confirmed by fluorescence microscopy using fluorescent brightner 28, which stains cell walls. About 88% of C. vulgaris K-73112 cells were covereted into osmotically-labile cells. Futhermore, a method for regeneration of intact cells from the protoplasts was developed. Utilization of 0.5M sucrose as an osmoticum, Fe-EDITA as an iron source, and bacto-agar as a supporting was shown to help regeneration of the cell walls of two strains, C. vulgaris K-73112 and C-27.続きを見る
|