Efficiency of additive agents was studied to remove copper from Cu-mixed soils by Brassica juncea and Zea mays L. The plants were grown in decomposed granite soil (Regosol) and volcanic ash soil (Andosol) for 3 weeks. To simulate actually-contaminated soil, copper contents of 25μg Cu g[-1] and 250μg Cu g[-1] were prepared for Regosol and Andsol, respectively. EDTA was found to be and exellent additive agent to raise the availability of Cu in soils. The efficient of EDTA on Cu absorption by plants was limited in Regosol. On the other hand, in Andosol the Cu accumulation in shoot of Z. mays for the 100mM EDTA treatment were 3.7 times larger than that for the 0mM EDTA treatment. The Cu absorption by B. juncea was not affected by the EDTA addition to Andsol. These results suggest that the effect of EDTA on the Cu absorption by plants significantly changes with the addtive concentration and with the types of soil and plant.