Laboratory of Horticultural Science, Division of Agronomy and Environmental Sciences, Department of Agro-environmental Sciences, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院環境農学部門農業環境科学
We attempted to induce unreduced gamete of Phalaenopsis by arresting the meiotic process with nitrous oxide gas (N_2O). Phalaenopsis amabilis and P. equestris (2n=2x=38) were treated with nitrous oxide gas under pressure for 0, 24, 48 and 68 hrs. The treatments for 24 and 48 hrs induced unreduced gamete in P. amabilis but those for 68 hrs was harmful for flower bud in P. amabilis. No 2n pollen was obtained in P. equestris. Thus, the treatment with N_2O may provide a new approach for Phalaenopsis breeding.