<紀要論文>
Characterization of a cloned chromosomal fragment affecting differentiation in Streptomyces azureus ATCC14921

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概要 We characterized a transformant BalA1 of thiostrepton-producing strain Streptomyces azureus ATCC14921, which carries an approximately 2.5kb chromosomal DNA fragment in high-copy-number plasmid. While ...strain BalA1 formed little aerial hyphae, its morphological defect was restored by cultivation with S. azureus, S. laurentii and S. coelicolor. Strain BalA1 strongly inhibited the growth of B. subtilis more so than its parent strain, and also inhibited the development of its parent and some Streptomyces strains with thiostrepton resistance. Futhermore, it induced S. coelicolor A3(2) to produce undecylprodgiosin at an early stage of growth, and to increase in the amount of actinorhodin production. The 2.5kb fragment contained two orfs, orf1 and truncated orf2. The deduced products were somewhat similar to phosphoserine phosphatase-like protein and N-terminal region of MinD-like protein, respectively. The individual function of orf1 or jointed function of both orfs seems to induce particular phenotypes or properties in strain BalA1.続きを見る

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登録日 2012.08.02
更新日 2021.02.05

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