<紀要論文>
Genetic diversity in Thai strains of Erwinia carotovora subsp. carotovora based on ERIC profiles and RFLP of a pel gene

作成者
本文言語
出版者
発行日
収録物名
開始ページ
終了ページ
出版タイプ
アクセス権
Crossref DOI
関連情報
概要 Molecular fingerprinting techniques of enterobacterial repetitive intergenic consensus (ERIC) and restriction fragment length polymorphism (RFLP) of a pel gene were applied to determine genetic divers...ity in Thai strains of Erwinia carotovora subsp. carotovora. ERIC fingerprinting revealed a greater genotypic diversity with 10 PCR groups in 22 Thai strains. When PCR for the pel gene was carried out using the four subspecies atroseptica, carotovora, betavasculorum and wasabiae, no PCR products were obtained in the case of betavasculorum. RFLP analysis with Sau3A was undertaken for the amplified fragments. Digestion patterns of 22 Thai strains were divided into two groups. ERIC group 1 and RFLP group 2 were composed of biovar D that were isolated from chinease cabbage, coriander and sweet pepper. The genetic diversity of E. carotovora subsp. carotovora appears to be related to their host plants and biovars in considering ERIC and RFLP data. These results indicate that ERIC and RFLP axalysis will be useful as benchmarks for genetic characterization of E. carotovora at subspecies and infraspecific level.続きを見る

本文ファイル

p015 pdf 1.23 MB 191  

詳細

PISSN
NCID
レコードID
査読有無
主題
ISSN
NCID
登録日 2012.08.02
更新日 2021.02.05

この資料を見た人はこんな資料も見ています