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Denazyme AP from Aspergillus oryzae was successfully encapsulated into liposomes by using the reverse-phase evaporation vesicle method. Liposomes were prepared with a yield of 52.0% and a particle dia...meter of several μm. The liposomes were very resistant to digestion in the gastric environment (pH 1.2), suggesting that liposome-encapsulation is an effective means for protecting denazyme AP against gastric conditions. Further, the efficiency of release was about 80% within 10min in a solution or bile salts (pH 6.2). To increase the quantity of denazymc AP encapsulated by this method, the optimal conditions for liposome-encapsulation were investigated. The encapsulation efficiency was improved by increasing the lecithin concentration in ethanol to 75mM. The ratio of lecithin/denazyme AP also affected the efficiency, giving the highest value at a ratio of 1.0.続きを見る
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