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Transformation of silage-making Lactobacillus strains by electroporation with plasmid vectors

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概要 Several newly isolated strains of Lactobacillus for silage fermentation were transformed with plasmid vectors, pGK12 and pSA3, by electroporation. Transformation efficiency depended on the electric fi...eld strength, time-constant, polyethyleneglycol (PEG) molecular weight in electroporation buffer and the incubation temperature after exposition of pulses. Transformation of L. pentosus NGR10225 resulted in the maximum transformation efficiency of 5.7x 10' transformants perμg DNA of pGK12 with an electric field strength of 7.0 kV/cm, time-constant of 6.2ms and 30% (W/V) of PEG 1000. Plasmid DNAs isolated from all transformants did not show any detectable rearrangements or deletions under these conditions.続きを見る

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登録日 2012.08.02
更新日 2021.02.05

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