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Histone-like protein of Streptomyces lividans

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概要 A DNA-bineting protein (about 10kDa and pI>9.7) of Streptomyces lividans TK24 was purified on a denatured DNA-Cellulose column, and then on a native DNA-Cellulose column. The N-terminal amino acid seq...uence of this protein had high homology with those of small basic DNA-binding proteins known as histone-like proteins. Thus, this protein was designated HSI (histone-like protein of S. lividans). Gel retardation assay revealed that HSI bound with the single-stranded DNA as replication intermediates of pSA1.1. We propose that HSI may participated in the replication of pSA1.1. The hup gene encoding HSI was cloned and sequenced. The deduced N-terminal amino acid sequence, molecular mass (9851 Da) and pI (9.95) were in good agreement with characteristics of HSI. HSI had the signature sequence for the histone-like proteins. Phylogenetic analysis suggested that HSI did not belong to the cluster of histone-like proteins from most of bacteria. The hup transcript of about 500 nucleotides was detected. The hup fragment hybridized with the AseI fragment C in the 9-10 o'clock region of the chromosome. Total DNAs of many Streptomyces species hybridized with the internal region of hup.続きを見る

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登録日 2012.08.01
更新日 2021.02.05

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