<紀要論文>
Protoplast Regeneration and Transformation of Thiostrepton-Producing Streptomyces Zaurentii ATCC 31255

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概要 The optimum condition for the protoplast formation and regeneration of S(yeptomyces Laurentii ATCC 31255 was studied. The effective formation and regeneration of protoplasts were obtained by using the... mycelia grown to the stationary phase in the presence of 0.4% glycine, and by treatment with 1 mg/ml of lysozyme in PWP buffer. When plasmids, pMCP5 and its derivative pYK3 (smaller in size) having a kanamycin resistance gene, were transformed into the protoplasts by using 20% (V/V) PEG 2000 in PWP buffer, the high number of transformants were obtained at a frequency of 4.3 x 10^5 and 3.4 x 10^4 per pg DNA of pMCP5 and pYK3, respectively.続きを見る

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登録日 2012.08.01
更新日 2021.02.05

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