second developments, respectively. Omission of the first step and the direct development with the second solvent gave worse results with tailing. Inter-genus differences, at least, were observed among the chromatograms. Striking difference of chromatogram was observed between Clavibacter spp. (Gram positive) and others (Gram negative). The chromatograms of Erwinia spp. were characteristic and quite different from those of Xanthomonas spp. and Agrobacterium tumefaciens. Although some difficulties like the cases of Pseudomonas spp. are still remaining, the practical usefulness of this method for a rapid identification of phytopathogenic bacteria was certainly verified.
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