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Bacillus subtilis (natto) Plasmid Responsible for Polyglutamate Production Encoding r-Glutamyltranspeptidase

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概要 Gamma-glutamyltranspeptidase (r-GTP) activity of PGA-producing strains derived by DNA-mediated transformation was significantly increased compared with that of protease, cu-amylase, and alkaline phosp...hatase in the PGA-nonproducing recipient strain, Bacillus subtilis Marburg 168. Its enzyme activity was deficient in the stringy-negative mutants cured with acridine orange. It is possible to deduce that r-GTP activity responsible for PGA production may be under the control of a specific regulatory gene(s) as extrachromosomal element. Parental strains were found to possess a plasmid, but PGA-nonproducing derivatives were missing the plasmid. The molecular weight of the plasmid were estimated to be 5.7 kilobase (kb) and restriction endonuclease cleavage site map was constructed. Therefore, we conclude that the function of the 5.7-kb plasmid detected in B. subtilis (natto) is concerned with PGA production. This observation suggests that a broader range of functions are associated with plasmid in Bacillus species.続きを見る

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登録日 2012.08.01
更新日 2020.11.02

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