<紀要論文>
Transfection and Transformation Systems for Pock-Forming and Thiostrepton-Producing Streptomyces azureus

作成者
本文言語
出版者
発行日
収録物名
開始ページ
終了ページ
出版タイプ
アクセス権
Crossref DOI
概要 To establish a procedure for high frequency transfection and transformation of Streptomyces azureus, at first, the condition for the protoplast formation and regeneration of this strain were studied. ...The effective formation and regeneration of protoplasts of S. azureus were obtained by using mycelia grown in the presence of 0.4 % glycine. The effective transfection of S. atureus protoplasts with temperate phage SAt 1 DNA was obtained at 20 % (W/V) PEG 2000. The frequency of transfection under the optimal condition was about 1 per 5×10^6 DNA molecule (equal to 5×10^3 transfectants per tig DNA) and 1 per 5.3×10^3 regenerated protoplasts. The DNA transfection of a virulent phage SAvl was also succeeded in the protoplasts of S. azureus. The transformation of pock-cured strain AF-6 of S. azureus with pock-inducing plasmid DNA occurred under the same condition as transfection of phage SAtl DNA.続きを見る

本文情報を非表示

p179 pdf 598 KB 144  

詳細

PISSN
NCID
レコードID
査読有無
関連情報
主題
ISSN
NCID
登録日 2012.08.01
更新日 2021.02.05

この資料を見た人はこんな資料も見ています