The spatial distribution of substances in a reacting and diffusing system has attracted a special interest of many investigators in connection with the operation of immobilized enzyme column, mechanism of enzymatic reaction under cellular conditions and so on. In the present study, the experimental results on the separation of α- and β-anomers of 2-acetamido-2-deoxy-D-glucopyranose with a partition column chromatography were analyzed by computer simulation, as an example of reacting and diffusing system.