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Transformation of Pseudomonas aeruginosa with Chromosomal DNA into Bacillus subtilis

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概要 Successful transformation of gram-negative and gram-positive bacteria was achieved. Genetic transformation of Pseudomonas aeruginosa KYU-1 to Bacillus subtilis Marburg 168 was performed with linear ch...romosamal DNA, provided the cells were treated with cold calcium chloride and a heat pulse. The molecular weight of transforming DNA of the donor was markedly dependent on the transformation frequency. At 6.2x10^6 daltons, transformation frequency with chromosomal DNA of P. aeruginosa into B. subtilis was obtained at 2.0x10-5. The divalent ions such as Ca^<2+> and Mg^<2+> enhanced transformation frequency.続きを見る

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登録日 2012.07.31
更新日 2020.10.26

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