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Sucrose-induced autolysis of Clostridium saccharoperbutylacetonicum (ATCC 13564) was inhibited by various chemical inhibitors, including known enzyme inhibitors (such as heavy metal ions and p-chlorom...ercuric benzoate) and fixative agents (such as formalin and glutaraldehyde). Fradiomycin (neomycin) also inhibited the lysis at the concentration of above 50pg/ml. The organisms whose growth had been inhibited by the antibiotics sucn as chloramphenicol, were resistant to sucrose-induced autolysis. The lysate produced by sucrose treatment had lytic activity, autolysin activity, on isolated cell wall. During the lysis, autolysin was released from the cells concomitantly with the degradation products of cell wall labelled with ^<14>C-D-alanine. In spite of these release of cell wall-degradation products and autolysin, the cell number and cellular content of macromolecules (DNA, RNA and protein) were hardly decreased. We suggest that sucrose-induced autolysis is due to the action of autolysin and progresses without destruction of protoplasmic membrane.続きを見る
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