<紀要論文>
Studies on Sucrose-Induced Autolysis of Clostridial Cells : Part 1. Induction of a Rapid Bacterial Autolysis by Sucrose Treatment

作成者
本文言語
出版者
発行日
収録物名
開始ページ
終了ページ
出版タイプ
アクセス権
概要 Growing cells of Clostridium saccharoperbutylacetonicum Nl-4 (ATCC 13564) were rapidly autolyzed in the presence of 0.3 to 0.5M sucrose. This autolysis was apparently induced by the treatment with suc...rose, and termed “sucrose-induced autolysis.” The maximum effect was shown at 0.35M sucrose, exhibiting about 50% loss of initial turbidity of the cell suspension. The rate of lysis depended on the age of culture. The most rapid lysis occurred in the cells of early-exponentially growing culture, hut no lysis was observed on those of late-exponential and stationary phase cultures. The optimal pH was 6.0. and the optimal temperature 30℃. Lysis occurred either in the cultural medium or buffer solution (for example: sodium phosphate buffer). The lysis was accompanied by striking morphological conversion from original rod cells (3 -6x0.4 -0.6 nrn) to spherical cells (1.0- 1.5 μm in diameter). The same rapid autolysis was also observed on the relative strains of C. succhuroperbutylacetonicum and C. sporogenes. Cells of C. butyricum. C. kaneboi and C. botulinurn were slightly autolyzed (10 - 15 % loss of initial turbidity). However, no lysis occurred on the aerobes of several different genus and on the cells of some other species of genus Clostridium tested. The bacterial spheres developed during the lysis may be the protoplasts.続きを見る

本文情報を非表示

p033 pdf 420 KB 210  

詳細

レコードID
査読有無
関連情報
主題
ISSN
NCID
登録日 2012.07.31
更新日 2020.11.02

この資料を見た人はこんな資料も見ています