This paper deals with screening and partial purification of a cytotoxic substance from the spleen of mice resistant to the Ehrlich ascites tumor. The purification was performed by a fractionation with ammonium sulfate, sucrose density-gradient centrifugation, and isoelectric focusing electrophoresis. The cytotoxicity was recovered in the microsomal fraction of the spleen extract. Thus, it was concluded that the cytotoxic substance was not humoral.
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