Transport of Thymidine inte the Developing Silk Gland as a Rate-limiting Step for the Formation of DNA Precursors. - 九大コレクション

＜紀要論文＞
Transport of Thymidine inte the Developing Silk Gland as a Rate-limiting Step for the Formation of DNA Precursors.

作成者	作成者名 Takechi, Kazuo 所属機関所属機関名 Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University
	作成者名 Koga, Katsumi 古賀, 克己所属機関所属機関名 Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University 九州大学農学部農芸化学科蚕糸化学講座
	作成者名 Hayashi, Katsuya 林, 勝哉所属機関所属機関名 Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University 九州大学農学部農芸化学科蚕糸化学講座
本文言語	英語
出版者	Kyushu University
出版者	九州大学農学部
発行日	1978-07
収録物名	九州大学大学院農学研究院紀要
巻	22
号	4
開始ページ	227
終了ページ	232
出版タイプ	Version of Record
アクセス権	open access
Crossref DOI	https://doi.org/10.5109/23669
関連DOI	九州大学農学部紀要 \|\| 22(4) \|\| p227-232
	Journal of the Faculty of Agriculture, Kyushu University \|\| 22(4) \|\| p227-232
	http://www.agr.kyushu-u.ac.jp/
関連URI	九州大学農学部紀要 \|\| 22(4) \|\| p227-232
	Journal of the Faculty of Agriculture, Kyushu University \|\| 22(4) \|\| p227-232
	http://www.agr.kyushu-u.ac.jp/
関連情報	九州大学農学部紀要 \|\| 22(4) \|\| p227-232
	Journal of the Faculty of Agriculture, Kyushu University \|\| 22(4) \|\| p227-232
	http://www.agr.kyushu-u.ac.jp/

ファイル	ファイルタイプ	サイズ	閲覧回数	説明
p227	pdf	115 KB	259

PISSN	0023-6152
NCID	AA00697606
レコードID	23669
査読有無	査読無
主題	DNA precursor
	Silk gland
	Thymidine
ISSN	0023-6152
NCID	AA00697606
注記	Developmental changes in the rate of thymidine uptake into the posterior silk gland of Bombyx mori was investigated by incubating the organ in a medium containing labelled thymidine. In the glands taken at day 2 of the 5th instar when the gland rapidly synthesizes DNA, the whole cell uptake of thymidine followed Michaelis-Menten kinetics with an apparent K'm of 25 μM. This value was larger than the measured concentration of thymidine (1 to 2 μM) in the hemolymph where the glands originally exist, and smaller than the Km of the silk gland thymidine kinase (for the thymidine substrate) as determined by a partially purified enzyme (3.3 μM). On the other hand, the older silk glands in which the rate of DNA synthesis is low (day 4 of the 5th instar) incorporated thymidine with kinetics less of the Michaelis-Menten type but in more of a linear manner; the apparent K'm value for the whole cell uptake of thymidine became as high as about 1 mM. These findings suggest that the uptake system of the silk gland for extracellular thymidine may serve as a rate-limiting step in the formation of steady state intracellular thymidylate pools.
登録日	2012.07.31
更新日	2022.11.11