九州大学大学院生物資源環境科学研究科生物機能科学専攻食品バイオ工学講座
Institute of Food Biotechnology, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School Kyushu University
九州大学大学院生物資源環境科学研究科生物機能科学専攻食品バイオ工学講座
Institute of Food Biotechnology, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School Kyushu University
九州大学大学院生物資源環境科学研究科生物機能科学専攻食品バイオ工学講座
Institute of Food Biotechnology, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School Kyushu University
A glassy carbon electrode, anodized for a short time at about 2.0 V vs. Pt，is a very convenient regenerable solid phase for the successive steps of an automated enzymelinked immunosorbent assay. The anodized surface absorbs allergen and the blocking proteins in a similar manner to classical polystyrene solid phase; the detection of the immobilized alkaline phosphatase used as a label can be achieved from an enzymatic process occurring; and the protein stacking, accumlated during the assay, can be eliminated by a simple re-anodization at the end of the cycle, allowing the adsorption of a new allergen. Using peroxidase or alkaline phosphatase as labels, various allergens such as ovalbumin, ovomucoid, and β-lactoglobulin were assayed.
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