On the multiplication of Cattleya species by shoot tip culture, some phenomena in which the medium changes into reddish brown and the growth of shoot tip is inhibited have occurred. Although the participation of phenolic compound is suggested, the reason is still unclear. In this study, eucomic acid and tyramine were isolated as major phenolics from Cattleya trianaei "Mooreana" and identified by spectrophotometric comparison. Eucomic acid was apparently effective at a concentration of 5ppm for the assay of growth inhibition using the shoot tip culture of Sophrolaeliocattleya(Slc) Jane Miyoshi "Gertie." Tyramine had no effect for the same bioassay, whereas the original fraction was rather strongly stimulatory. It was suggested that some growth stimulator should be existent in this fraction. In the light of the present result, the formations of eucomic acid and tyramine may play an important role in the metabolism of amino acid and TCA cycle. The biogenetic pathways of eucomic acid including tyramine have been proposed.
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